AN ACCURATE FORMULA FOR USE IN THE MODIFICATION OF COW'S MILK IN THE ARTIFICIAL FEEDING OF INFANTS
Journal of the American Medical Association (JAMA)
Litmus lactose gelatin plates were kept three days at room temperature, then »examined and counted. Lilian.---laeiose-agar plates were incubated at 37 C. for twenty-four hours before »examination. Blood-agar plates were kept three days at 37 C. under anaerobic conditions, secured by passing hydrogen through Now jars containing the plate »cultures, then extract¬ ing the last traces of oxygen with pyrogallie acid and sodium hydrate. Examinations at the end of three days were made and especial
... de and especial attention paid to bemolytic powers of the developing colonies. Oval colonies surrounded by distinct bemolyt ic zones developing from spores in blood-agar corresponded mor¬ phologically and culturally with /,'. aerogehes capsulatus or H. perfringens. Subcultures were made from typical colonies in each stool and furl her study of these confirmed the first impression that the large majority were II. uermjenes cap¬ sulatus. Another paper, purely bactériologie in nature, dealing with all the varieties of spore-forming anaerobic bacteria that have been found, may be published later, and for this reason only such points arc mentioned in Ibis article as bear on the clinical aspects of the relationship bel ween joints and gastro-intestinal disorders.