MOLECULAR ANALYSIS OF GENETIC STABILITY OF MICROPROPAGATED BLACKBERRY AND BLUEBERRY PLANTS USING RAPD AND SRAP MARKERS
DETERMINAREA STABILITĂȚII GENETICE A MATERIALULUI SĂDITOR DE AFIN ȘI MUR OBȚINUT PRIN MICROPROPAGARE CU AJUTORUL MARKERILOR MOLECULARI RAPD ȘI SRAP

Doina Clapa, Institute of Advance Horticulture Research of Transylvania, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Romania, Orsolya Borsai, Monica Hârța, Radu Cristian Sisea, Doru Pamfil, Institute of Advance Horticulture Research of Transylvania, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Romania, Life Sciences Institute, Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Romania, Institute of Advance Horticulture Research of Transylvania, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Romania, AgroTransilvania Cluster, Dezmir, Crișeni FN, Cluj-Napoca, Romania; University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Romania
2019 Fruit Growing Research  
Micropropagation of blackberry (Rubus fruticosus L.) and blueberry (Vaccinium corymbosum L.) plants is a successful alternative for plant material production. Among the micropropagation methods developed axillary bud stimulation is the most commonly used method for mass propagation of different plants and considered the most reliable method to guarantee genetic stability of the regenerated plants obtained by micropropagation. Although, a major problem of this type of propagation method is the
more » ... ion method is the somaclonal variation which can occur among the subcultured plants of the parental line, especially after a high number of subcultures. Therefore, the aim of this research was to evaluate the genetic stability of the micropropagated plants of two blackberry cvs. 'Loch Ness' and 'Chester Thornless' and three blueberry cultivars ('Aurora', 'Draper' and 'Liberty') using RAPD and SRAP markers. Our results reveal that, no genetic variations were shown amongst the mother plants and micropropagated plants of the investigated species providing thus further evidence for the genetic stability. Cuvinte cheie: micropropagare, markeri moleculari, uniformitate genetică.
doi:10.33045/fgr.v35.2019.12 fatcat:unoem3sfqzbz5kyalc3j4dwusa