The American mathematical monthly
Sea anemone (Cnidaria, Anthozoa) venom is an important source of bioactive compounds used as tools to study the pharmacology and structure-function of voltage-gated K+ channels (Kv). These neurotoxins can be divided into four different types, according to their structure and mode of action. In this work, for the first time, two toxins were purified from the venom of Bunodosoma caissarum population from Saint Peter and Saint Paul Archipelago, Brazil. Sequence alignment and phylogenetic analysis
... logenetic analysis reveals that BcsTxl and BcsTx2 are the newest members of the sea anemone type 1 potassium channel toxins. Their functional characterization was performed by means of a wide electrophysiological screening on 12 different subtypes of Ky channels (K vl.l-K vl.6; Ky2.1; Ky3.1; Ky4.2; Ky4.3; /?ERG and Shaker IR). BcsTxl shows a high affinity for rKvl.2 over rKvl.6, Mar. Drugs 2013, 11 656 hKvl.3, Shaker IR and rK vl.l, while Bcstx2 potently blocked rKvl.6 over hKvl.3, rK vl.l, Shaker IR and rKvl.2. Furthermore, we also report for the first time a venom composition and biological activity comparison between two geographically distant populations of sea anemones.