Structural Organization and Chromosomal Assignment of the Swine Endothelin-1 Gene

Misaki KOJIMA, Kousuke OHATA, Hiroshi YASUE
1997 Journal of Veterinary Medical Science  
Cosmid clone containing swine endothelin-1 (EDN1) gene, cosEDN1, was isolated from swine cosmid library using swine EDN1 cDNA as a probe. The sequence analysis of cosEDN1 DNA revealed that the swine EDN1 gene consists of 5 exons, spanning approximately 6.5 kb. In the 5'-upstream region of the EDN1 gene, AP-1 and NF-1 elements were found, suggesting the possibility that the expression of swine EDN1 gene is controlled by protooncogene products Fos and Jun, and TGF-β. Fluorescence in situ
more » ... ce in situ hybridization (FISH) using cosEDN1 DNA as a probe demonstrated that EDN1 gene resides on swine chromosome 7p13->pter. -KEY WORDS: EDN1, FISH, gene structure, mapping, swine.
doi:10.1292/jvms.59.431 pmid:9234216 fatcat:5ktvbcm54nforjueamnf7rnh54