A Method for Analysis of Cell to Cell Transmission of Theileria sergenti Piroplasms

Yutaka TERADA, Masayoshi TSUJI, Yurie NAKAMURA, Katsuro HAGIWARA, Kiyoshi TAKAHASHI, Chiaki ISHIHARA
1998 Journal of Veterinary Medical Science  
We tried to develop an experimental system for quantifying piroplasm transmission in Theileria sergenti infection using a SCID mouse model, of which red blood cells (RBC) had been substituted with bovine (Bo). A mouse hybridoma cell producing antibody against Holstein RBC was established for distinguishing Holstein RBC from Japanese Black RBC by a immuno-staining method. T. sergenti piroplasms in RBC were stained by 4',6-diamidino-2-phenylindole. With the aid of these techniques the piroplasm
more » ... ues the piroplasm transmission from one to another race of Bo-RBCs could successfully be quantified in SCID mice. -KEY WORDS: erythrocyte, Theileria sergenti, transmission.
doi:10.1292/jvms.60.115 pmid:9492371 fatcat:rkyjyzgxofhtvgcjtjl4pqblpy