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Commercial lactate oxidase (Lactate:O2; oxidoreductase EC 184.108.40.206) from Pediococcus species was immobilized on to alkylamine glass beads (pore diameter 55 nm) through glutaraldehyde coupling with a conjugation yield of 3.2 mg/g support and 105% retention of initial activity. Immobilized enzyme showed maximum activity at pH 6.5, when incubated at 40 degrees C for 12 min and was used for determination of lactic acid in serum. The H2O2 generated from serum lactate by immobilized enzyme waspmid:23923563 fatcat:bmes37m4qzagjal3wkqszpllr4