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Aim: Methodology: Results: Interpretation: Keywords: This study explores the possibility of treating basic dye, methyl orange using Scirpus grossus in a batch free flow surface system. The effect of operational parameters (pH, ORP, ABS, BOD, COD and TOC) for different dye -1 concentrations (0, 200, 400, 600, 800 and 1000 mg l ) were observed and determined during 42 days. The results of UV-Vis absorption, HPLC and GC-MS confirmed the degradation of methyl orange. The removal efficiency ofdoi:10.22438/jeb/40/3(si)/sp-16 fatcat:pq6avvpqmzhutbatbkx6qv2d4i