ゲル作成時の温度がDGGEのバンドラインの歪みに及ぼす影響
Temperature conditions on casting a gel minimizing the band distortion of DGGE

Yuko Matsushita, Sho Morimoto, Chika Bao, Seya Tsushima
2010 Tsuchi to biseibutsu  
Fbr denaU ユring gradient gel electrophoresis ( DGGE ) analysis , the band distortion has been known to be afEected by the unevenness of an electric current and the bu丘er temperature during DNA loading . However, 1ittle is known about the ef[ ect of the temperature on the band distortion 、 The eflect of the temperatures of the gel solution ( denaturing gel solution temperature :DGT ) and glass plates ( plate temperature :PT ) at the gel − pouring time was examined in the range of 5 − 28℃ and 10
more » ... of 5 − 28℃ and 10 − 28℃ , respecli ▽e1 } 孔 The results showed that the band distortion increased as the difFerence between DGT and PT increased when tbe same DNA sQlutions were loaded in all wells . From these results , the combination of DGT and PT was considered to be a crucial factor for reducing the 1〕and distortion . These results and the study of the relationship between the gel polymerization rate and the cQmbination of the two temperature $ in an in vitro experiment suggested the heterogeneity of the polymerization rate within a glass plate.
doi:10.18946/jssm.64.2_107 fatcat:gj7xhsfxnjhkfnozo6zbk33ksi