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The objective of this research was to determining of protein from connective tissue (protein) in the inner layer of organic and broiler chicken gizzard. The presence of collagens was observed by submersion with 0,1 M NaOH base pH 8 and without submersion. The physiochemical properties and characteristic of collagen from inner layer chicken gizzard such as FTIR spectra, element content, morphology and SDS-PAGE pattern were evaluated. FTIR spectrum in the sample without submersion shown collagendoi:10.1088/1742-6596/694/1/012074 fatcat:v4h6ohgzvfd6pkwg2lwlmy2s4m