Understanding the spatial ecology of animals is an important part of 16 conserving biodiversity. In this context, manual radio-tracking is a primary means for 17 understanding the movement of freshwater fish. Our aim was to determine the 18 suitability of using linear or area-based estimates to quantify diel use of space by 19 Macquarie perch, Macquaria australasica (Percichthyidae), in a reservoir. Area-based 20 estimates were calculated as minimum convex polygons (MCPs). Four-hourly radio-21

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... racking of 23 adults within diel periods resulted in 136 pairs of linear and area-based 22 Additional keywords: bias, minimum convex polygon, radio-tracking, threatened 34 species, Percichthyidae, reservoir. 35 36 Running headline: diel range estimation 37 38