Comprehensive analysis of hepatic gene and protein expression profiles on phenobarbital- or clofibrate-induced hepatic hypertrophy in dogs

Toshihiko Makino, Junzo Kinoshita, Shingo Arakawa, Kazumi Ito, Yosuke Ando, Takashi Yamoto, Munehiro Teranishi, Atsushi Sanbuissho, Hiroyuki Nakayama
2009 Journal of Toxicological Sciences  
® analysis and proteomics analysis using the 2-dimension difference gel electrophoresis (2D-DIGE) technique, were performed. Both compounds caused centrilobular hepatocellular hypertrophy, which were related to smooth endoplasmic reticulum (SER) proliferation in PB-treated dogs and to mitochondrial prolifer-ed dogs, drug-metabolizing enzyme induction was not clearly observed by any of Western blot, genomic and proteomic analyses. Genomic and proteomic analyses revealed that mitochondrial genes
more » ... itochondrial genes and proteins, nase, pyruvate carboxylase and ATP synthase beta chain were induced. There is a relatively good correlation among the morphology and the genomic and proteomic data, but some differences exist between the ical evaluation may provide a useful tool for safety assessment of the liver. Dog Toshihiko Makino
doi:10.2131/jts.34.647 pmid:19952500 fatcat:7ygf4ky2mre47jt4yy5lkysley