In vitro study of Tinospora cordifolia (Willd.) Miers (Menispermaceae)

Aditi Singh, Saroj K Sah, Aunji Pradhan, Sabari Rajbahak, Niran Maharajan
2010 Botanica Orientalis Journal of Plant Science  
In vitro study was carried out in an important medicinal plant Tinospora cordifolia (Willd.) Miers belonging to the family Menispermaceae. Vegetative parts such as stem, leaf and nodal explants were excised from an elite in vivo grown mature plant and thereafter cultured on Murashige-Skoog (MS) medium supplemented with different hormonal concentrations for callus induction and organogenesis. Callus formation occurred from nodal segments, leaf and inter-node explants when planted on different
more » ... binations of hormones. Tinospora cordifolia showed response for in vitro shoot growth from the nodal segment. The best shoot growth was observed on MS medium supplemented with kinetin (1.5 mg/l). Similarly, the best result for root induction was obtained on MS medium supplemented with 6-benzylaminopurine (1.0 mg/l) and naphthaleneacetic acid (2.5 mg/l).
doi:10.3126/botor.v6i0.2918 fatcat:6wxggjh54na2xltn4nfshgkex4