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This paper reports on-chip based optical detection with threedimensional spatial resolution by integration of an optofluidic microscope (OFM) in a microfluidic pinched flow fractionation (PFF) separation device. This setup also enables on-chip particle image velocimetry (PIV). The position in the plane perpendicular to the flow direction and the velocity along the flow direction of separated fluorescent labeled polystyrene microspheres with diameters of 1 μm, 2.1 μm, 3 μm and 4 μm is determineddoi:10.1364/oe.18.004158 pmid:20389429 fatcat:uuifpvko4vacdgl3dx4rhgu25e