Efecto antimicrobiano de la terapia fotodinámica sobre Enterococcus faecalis, estudio in vitro

Emmanuel João Nogueira Leal Silva, Wagner Pereira Coutinho-Filho, Aurimar de Oliveira Andrade, Daniel Rodrigo Herrera Morante, Raphael Hirata-Junior, Tauby de Souza Coutinho-Filho, Renato Liess Krebs
2014 Revista Estomatológica Herediana  
Efecto antimicrobiano de la terapia fotodinámica sobre Enterococcus faecalis, estudio in vitro Revista Estomatológica Herediana, vol. 21, núm. 4, octubre-diciembre, 2011, pp. 185-189 Universidad Peruana Cayetano Heredia Lima, Perú Disponible en: http://www.redalyc.org/articulo.oa?id=421539366002 Cómo citar el artículo Número completo Más información del artículo Página de la revista en redalyc.org Sistema de Información Científica Red de Revistas Científicas de América Latina, el Caribe, España
more » ... , el Caribe, España y Portugal Proyecto académico sin fines de lucro, desarrollado bajo la iniciativa de acceso abierto Palabras clave: Enterococus faecalis / FOTOQUIMIOTERAPIA / INFECCIÓN ENDODÓNTICA. Antimicrobial effect of photodynamic therapy against Enterococcus faecalis, in vitro ABSTRACT OBJECTIVE: To evaluate in vitro, the antibacterial effect of photodynamic therapy (PDT) against Enterococcus faecalis. MATERIAL AND METHODS: Bacterial suspensions were prepared from clinical strains of E. faecalis. These suspensions were divided into three experimental groups (1L, 2L and 3L) and a control group (CG). The Twin Laser equipment with a GaAIAs diode was used to expose experimental groups to PDT as follow: 1 min at 60 J/cm2, 2 min at 120 J/cm2 and 3 min at 180 J/cm2. After the experimental treatments, 0.1 mL aliquots of the suspensions were diluted, seeded and incubated for further counting of Colony Forming Units per mL (CFU/mL). The results were analyzed by analysis of variance and Tukey's test (p<0.05). RESULTS: The groups 1L, 2L and 3L showed a reduction of 99.88%, 99.86% and 99.92% respectively (p<0.05). However, no signifi cant differences were found between experimental times of PDT (p>0.05). CONCLUSION: The results showed that PDT was effective in reducing the number of viable cells of E. faecalis, a microorganism frequently associated with failure of root canal failure.
doi:10.20453/reh.v21i4.164 fatcat:rm2iheztj5cijj5lvbi6o6ndka