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Many theoretical models have been proposed to study the effect of space on population dynamics and interactions, but most of them are difficult to translate into experimental setups due to their abstract nature. Here we defend the gradostat as a valuable experimental tool for testing such theories. The gradostat is a culture system with bi-directional flow that forms nutrient gradients at steady state. In this study, we use a 3-vessel gradostat with a phosphate gradient to study the effect ofdoi:10.1590/s0034-71082000000400014 fatcat:qjssitcjkrdqnde6hio77caj3e