Morphological, histological and molecular evidence of Myxobolus spinacurvatura (Cnidaria: Myxosporea) from Mugil cephalus in the Turkish Black Sea coast

2020 Turk veterinerlik ve hayvancilik dergisi  
3 In the present study, a total of thirty M. cephalus captured from the Samsun coast of the 4 Black Sea, Turkey were examined for myxozoan infections. Different sizes of whitish and 5 rounded cyst-like plasmodia were macroscopically detected in the liver and intestine wall 6 of M. cephalus. Morphological and histological examinations were performed. The SSU 7 rDNA was amplified using nested PCR using myxosporean-specific primers. The present 8 Myxobolus species was identified as M.
more » ... as M. spinacurvatura based on the spore morphology 9 and host/organ specificity. In the present study, morphological, histological and molecular 10 data about M. spinacurvatura were presented. Furthermore, the BLASTn research showed 11 that SSU rDNA sequences of M. spinacurvatura (accession number MH374629) were 12 identical to that of the reference sequence of M. spinacurtavura (accession from 13 AF378341) recorded previously. The current study includes the first record and molecular 14 evidence of M. spinacurvatura infecting M. cephalus from the Turkish waters. To date, 15 there is no report on comprehensive morphological, histopathological and molecular data 16 of M. spinacurvatura in M. cephalus as a typical host. Moreover, the new valid SSU rDNA 17 sequence of M. spinacurvatura (accession number MH374629) was the second record in 18 the Genbank. The present SSU rDNA sequence can also be used to construct a phylogenetic 19 tree with other mugiliform-infecting Myxobolus species worldwide. 20
doi:10.3906/vet-1906-59 fatcat:bwnjutxopnbefbhs72huosjyw4