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Phytochemical investigations on the chloroform extract of the arial part of Orthosiphon stamineus resulted in isolation of one new prenylated compound 5,7-dimethoxy-3,4′-dihydroxy-3′,8-di-C-prenylflavanone together with four known flavonoids compounds, sinensetin, eupatorin, 5,6,7,4¢-tetramethoxyflavone and 3-hydroxy-5,6,7,4¢-tetramethoxyflavone. The structures were deduced on basis of different analytical methods such as UV, IR, 1H-NMR, 13C-NMR, DEPT, COSY, HMBQ and GC-MS. The prenylateddoi:10.22146/ijc.21661 fatcat:imd4gytjdfd3hfqhgt4jkiuf6e