Macrophages in Muscle Layer of Gastrointestinal Tract: Impairment of Muscle Contraction by Treatment with Lipopolysaccharide

Shigeko Torihashi, Masatoshi Hori, Hiroshi Ozaki
2001 Acta Histochemica et Cytochemica  
The morphology and function of resident macrophages in the muscle layer and in the subserosa of the gastrointestinal tract are described. FITC-dextran injected via the tail vein of a mouse or rat demonstrated a regular arrangement of macrophages on a whole mount preparation of the muscle layer. Electron microscopy revealed inactive phagocytosis under normal conditions. After a 4-8 hr incubation of rat ileal muscle with lipopolysaccharide (LPS) to investigate its effect on muscle contraction,
more » ... cle contraction, macrophages began to express inducible nitric oxide synthase (iNOS) and released nitric oxide. This macrophage response was accompanied by the expression of cyclooxygenase-2 (COX-2) immunoreactivity and the production of prostaglandins. The muscarinic agonist-induced contrac-tionwasgreatlyinhibitedbyLPStreatment, and this inhibition was reversed by either iNOS or COX-2 inhibitors. We concluded that one of the functions of muscular macrophages was the modulation of smooth muscle contraction under pathological conditions. The mechanism of iNOS upregulation indicated by our experiment is discussed.
doi:10.1267/ahc.34.219 fatcat:66qgblywfrhohafqcmyffkntke