Screening of historical isolates stored in New Zealand culture collections for Pseudomonas syringae pv actinidiae

D. Park, D. Than, K.R. Everett, J. Rees-George, M.K. Romberg, B.J.R. Alexander
2011 New Zealand Plant Protection  
Subsequent to the detection of Pseudomonas syringae pv actinidiae (Psa) on kiwifruit in Te Puke (November 2010) isolates of Pseudomonas spp from kiwifruit stored in the International Collection of Microorganisms from Plants (ICMP) and at Plant Food Research (PFR) were tested by PCR for Psa A total of 139 isolates from ICMP were assayed with the previously published primer pairs PsaF1/PsaR2 and PAV1/P22 An amplicon of expected size for Psa was produced from 19 isolates using primers PAV1/P22 but
more » ... rimers PAV1/P22 but no amplicons were obtained using the primers PsaF1/PsaR2 Amplicons produced by primers PAV1/P22 were sequenced and these isolates were identified as Pseudomonas viridiflava A further 52 Pseudomonas isolates collected from kiwifruit and stored at PFR did not amplify a PCR product with primers PsaF1/PsaR2 These PCR tests confirm that Psa was not detected in any of the cultures held in the ICMP or at PFR The screening of historical Pseudomonas isolates associated with kiwifruit suggests that Psa strains had not been isolated from New Zealand kiwifruit samples prior to the detection in 2010
doi:10.30843/nzpp.2011.64.6003 fatcat:b7mebyfkkjfalk5lrgfrke6xn4