Isoflavones and biotransformed dihydrodaidzein production with in vitro cultured callus of Korean wild arrowroot Pueraria lobata
한국산 야생칡 캘러스에서의 이소플라본 및 생물전환에 의한 디하이드로다이드제인 분석

Eunji Lee, Jung Eun Kwon, Soojung Kim, Min-Seok Cha, Inhye Kim, Se Chan Kang, Tae-Ho Park
2013 Journal of Plant Biotechnology  
Pueraria lobata, a medicinally important leguminous plant produces various isoflavones including puerarin, daidzin and daidzein which are metabolized to equol via dihydrodaidzein and tetrahydrodaidzein by the bacterial fermentation of natural isoflavone sources in human intestines. In this study, we described callus proliferation and isoflavone production in callus of Korean wild arrowroot and dihydrodaidzein biosynthesis in callus extract fermented with Pediococcus pentosaceus. Proliferation
more » ... us. Proliferation was the best at callus cultured in the medium containing 1.0 mg/L TDZ and 1.0 mg/L NAA at light condition for 12 days. Puerarin was significantly more produced at callus cultured in the medium containing 2.0 mg/L kinetin and 1.0 mg/L NAA at dark condition for 16 days, but daidzin and daidzein were not significant. Callus extract was successfully fermented with P. pentosaceus and dihydrodaidzein, which is one of equol precursors formed by biotransformation, was confirmed to
doi:10.5010/jpb.2013.40.4.217 fatcat:iqrk6rohpjgzzgejesuphwvw3q