GAS2 encodes a 2-oxoglutarate dependent dioxygenase involved in ABA catabolism [article]

Theo Lange, Nadiem Atiq, Maria João Pimenta Lange
2022 bioRxiv   pre-print
AbstractLiu et al.1recently reported the characterization ofArabidopsis thalianaGAS2 (Gain of Function in ABA-modulated Seed Germination 2), which was described as an enzyme that catalyzes the stereospecific hydration of GA12to produce GA1216, 17-dihydro-16α-ol (DHGA12). However, as previously reported2, we did not find conversion of [17-14C]-labeled or [1-,7-,12-,18-14C4]-labeled GA12by GAS2. Furthermore, the authors1state the isolation of endogenous DHGA12from dry Arabidopsis seeds, which we
more » ... annot confirm by our attempts to isolate this compound from 0.5 g dry Arabidopsis Col-0 seeds (data not shown). Instead, we present here data showing that the recombinant GAS2 enzyme is able to catabolize abscisic acid (ABA) to phaseic acid (PA) and further to a second product, putative 8'-carboxy-ABA (Fig. 1a).
doi:10.1101/2022.11.16.516706 fatcat:ytwbghgwejhlnoy36udipnkfnq