Development of Analytical Methods of Hyperoside from Rosa canina L
Rosa canina L. 중 hyperoside의 시험법 개발 연구

Jae Myoung Oh, Hwa Jung Lee, Kyeong Nyeo Bahn, Il Won Seo, Young Joo Lee, Jin Hee Lee, Ji Min Park, Tae Seok Kang
2015 Journal of Food Hygiene and Safety  
Rosa canina L. is health functional food materials that can help to temporarily relieve symptoms of arthritis. This study has been conducted to develop and validate analytical methods for hyperoside of Rosa canina L.. Methods based on HPLC with ultraviolet detection (UVD) were established through instrumental analytical conditions, and the examination of data, such as domestic and foreign reliable methods and journals. HPLC UVD analysis using Capcell Pak C 18 MG II column at 353 nm was
more » ... 353 nm was determined on test through the column, mobile phase. The validation has been performed on the method to determine linearity, accuracy, limits of quantification (LOQ) and repeatability for hyperoside. The method showed high linearity in the calibration curve at a coefficient of correlation (R 2 ) of 0.999, and the LOQ was 0.393 µg/mL. Relative standard deviation (RSD) values of data from repeatability precision was between 0.6 and 2.6%. Recovery rate test at hyperoside scored between 98 and 99%. These results indicate that the established HPLC method is very useful for the determination of hyperoside in Rosa canina L. to develop a health functional material.
doi:10.13103/jfhs.2015.30.2.173 fatcat:3w2zmomr6zawxjdpr7eqh7tmje