RNA extraction from wastewater for detection of SARS-CoV-2 v1 [post]

David Findlay, Julie Bolland, Brindusa Cerghizan, Kirsty Campbell, David Thomson, Alexander Corbishley, David Gally, Stephen Fitzgerald, Alison Tidswell, Sean McAteer, Livia C T Scorza
As part of the global response to the 2019 novel Coronavirus (SARS-CoV-2) pandemic, it was determined that SARS-CoV-2 RNA was detectable in the faeces of both symptomatic and asymptomatic patients (1). Further analysis demonstrated that a wastewater epidemiological (WWE) approach, similar to that used to track other viruses (i.e. Poliovirus), could be employed to monitor the spread of SARS-CoV-2. The presence of, or changes in concentration of viral RNA within the wastewater network can assist
more » ... n monitoring the emergence of further viral peaks (2). Thus, monitoring the spread of Covid-19 using the WWE approach has been extensively explored in several countries (3). This procedure, developed by the Scottish Environment Protection Agency (SEPA) based upon work of the Corbishley group at the Roslin Institute, University of Edinburgh, outlines the method for the concentration of viral RNA in wastewater, as well as in other types of environmental and potable water samples. In this method, a known volume of sample is concentrated using a centrifugal filter to allow further extraction and detection of SARS-CoV-2 RNA. For further analysis using RT-qPCR please search for "RT-qPCR for detection of SARS-CoV-2 in wastewater" by the same authors of this protocol on protocols.io References: 1. Jones, D. L., Baluja, M. Q., Graham, D. W., Corbishley, A., McDonald, J. E., Malham, S. K., Hillary, L. S., Connor, T. R., Gaze , W. H., Moura , I. B., Wilcox, M. H., & Farkas , K. (2020).Shedding of SARS-CoV-2 in feces and urine and its potential role in person-to-person transmission and the environment-based spread of COVID-19.Science of the Total Environment.https://doi.org/10.1016/j.scitotenv.2020.1413644. 2. Fitzgerald, S., Rossi, G., Low, A., McAteer, S., O'Keefe, B., Findlay, D., Cameron, G. J., Pollard, P., Singleton, P. T. R., Ponton, G., Singer, A. C., Farkas, K., Jones, D., Graham, D. W., Quintela-Baluja, M., Tait-Burkard, C., Gally, D., Kao, R., & Corbishley, A.(2021).Site specific relationships between COVID-19 cases and SARS-CoV-2 viral load in wastewater treatment plant influent. Environmental Science and Technology. https://doi.org/10.1021/acs.est.1c05029 3. Wastewater SARS Public Health Environmental Response (W-SPHERE). https://sphere.waterpathogens.org/about
doi:10.17504/protocols.io.bzv5p686 fatcat:puq2lh66nbfjzbwyhzxiehg37a