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The assembly of functional cellulolytic enzymes was displayed using a synthetic, cell-surface engineered diploid yeast consortium. Trichoderma reesei endoglucanase II (EGII), cellobiohydrolase II (CBHII), and Aspergillus aculeatus β-glucosidase I (BGLI) were displayed as fusion proteins with the AGA2p C-terminus of a-agglutinin on the cell surface of the diploid yeast strain Saccharomyces cerevisiae Y5. The cell-surface immobilization of each enzyme was confirmed by immunofluorescencedoi:10.15376/biores.10.3.4460-4472 fatcat:ndednjedxva3fozmrbpv5r2vju