Effects of Peroxisome Proliferator-Activated Receptor-γ on the Production of Tumor Necrosis Factor-α in Stimulated Human Monocoyte

Eun-Young Kwon, Chulmin Park, Jae-Cheol Kwon, Si-Hyun Kim, Sun Hee Park, Su-Mi Choi, Dong-Gun Lee, Jin-Hong Yoo, Jung-Hyun Choi
2010 Infection & Chemotherapy  
We evaluated the effects of peroxisome proliferator-activated receptor-γ (PPAR-γ ) on the production of tumor necrosis factor-α (TNF-α ) and expression of nuclear factor-κ B (NF-κ B) in stimulated THP-1 cells, a human monocyte cell line. Materials and Methods: We evaluated the cytotoxic effect of 15-Deoxy-Δ 12,14prostaglandin J 2 (15d-PGJ 2 ), one of natural PPAR-γ ligands, using commercial cell proliferation assay. Cells were pretreated with 15d-PGJ 2 and then stimulated with
more » ... h lipopolysaccharide (LPS) or lipoteichoic acid (LTA). The amount of TNF-α was measured by using commercial ELISA method. NF-κ B activation was evaluated by Western blot analysis. Results: 15d-PGJ 2 showed dose-dependent cytotoxic effect on the tested cells after 4 hr of treatment. Stimulation of cells by LPS or LTA induced TNF-α production. TNF-α production was markedly decreased in the cells pretreated with 15d-PGJ 2 compared to cells treated only with LPS or LTA in a dose-dependent manner. Pretreatment of 15d-PGJ 2 reduced LPS or LTA induced NF-κ B expression in the nuclear extracts of THP-1 cells. Conclusion: 15d-PGJ 2 pretreatment decreased TNF-α production from the THP-1 cells stimulated by LPS or LTA, and this assumed to be associated with inhibition of NF-κ B activation.
doi:10.3947/ic.2010.42.5.291 fatcat:el3odlzuqbdknitc4tprjoxxzm