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Cell staining CEM cells (1.7 × 10 6 cells) were diluted in 2 mL WB. CellTracker Green (2 μL; Invitrogen) was incubated with cells for 45 min at room temperature, according to manufacturer's instructions. The cells were centrifuged at 970 rpm for 3 min to remove excess dye, resuspended in 1 mL WB, and incubated for 30 min at 37°C. The cell solution was centrifuged at 970 rpm for 3 min, and the supernatant liquid was discarded. The pellet was washed with 1 mL of BB2, centrifuged at 970 rpm for 3doi:10.1039/c0mb00311e pmid:21424012 fatcat:qauf5maxmrhs3p75rs3p75ymgq