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Crystallographic and Kinetic Evidence of a Collision Complex Formed during Halide Import in Haloalkane Dehalogenase†
Crystallographic and kinetic evidence of a collision complex formed during halide import in haloalkane dehalogenase Pikkemaat, Mariël G.; Ridder, Ivo S.; Rozeboom, Henriette; Kalk, Kor H.; Dijkstra, Bauke W.; Janssen, Dick ABSTRACT: Haloalkane dehalogenase (DhlA) converts haloalkanes to their corresponding alcohols and halide ions. The rate-limiting step in the reaction of DhlA is the release of the halide ion. The kinetics of halide release have been analyzed by measuring halide binding withdoi:10.1021/bi990849w pmid:10508409 fatcat:xl43gq5skvbvtfymbw3hbeto5m