Chemical composition and anti-arthritic activity of the essential oil from Litsea cubeba against Type II collagen rheumatoid arthritis in rat collagen

Jiancun Zhao, Qianqian Wang, Jie Ma
2020 Tropical Journal of Pharmaceutical Research  
Purpose: To study the curative effect of the volatile oil from Litsea cubeba (EOL) on type II collagen (CII) induced arthritic (CIA) rat. Methods: The chemical constituents of EOL were analyzed by gas chromatography-mass spectrometry (GC-MS). The analgesic effect of the oil was assessed by acetic acid-stimulated torsion and hot plate methods, while antiinflammatory potential was further assessed by in an ear oedema model induced by dimethylbenzene in mice. The anti-rheumatoid arthritis (RA)
more » ... arthritis (RA) activity of EOL in mice was evaluated in terms of paw volume, arthritis index, thymus and spleen index, and serum inflammatory factor levels. Results: GC-MS showed that α-citral (26.42 %), β-citral (21.94 %), and limonene (12.79 %) were the main components of EOL. Different doses of EOL (50, 100, 200 mg/kg) exerted varying inhibitory effects on torsion in mice induced by acetic acid (p < 0.01) but had no significant effect on thermal stimulation-induced pain. EOL reduced ear oedema in mice (p < 0.01). In addition, EOL (50, 100, 200 mg/kg) reduced the mouse paw volume, arthritis index, and thymus and spleen index (p < 0.01). Furthermore, EOL reduced proinflammatory cytokines in serum but increased antiinflammatory cytokines (p < 0.01). Conclusion: EOL ameliorates symptoms of inflammation in CIA rats by inhibiting inflammatory reactions, suggesting it could be further developed as an anti-arthritic drug. Keywords: Litsea cubeba, Essential oil, Rrheumatoid arthritis, Pro-inflammatory cytokines
doi:10.4314/tjpr.v19i3.27 fatcat:m22mipvgn5atreczegesusvsye