Long-Term Exercise Modulates Hippocampal Gene Expression in Senescent Female Mice

María Jesús Alvarez-López, Marco Castro-Freire, Marta Cosín-Tomás, Sandra Sanchez-Roige, Jaume F. Lalanza, Jaume del Valle, Marcelina Párrizas, Antonio Camins, Merce Pallás, Rosa María Escorihuela, Perla Kaliman
2013 Journal of Alzheimer's Disease  
The senescence-accelerated SAMP8 mouse is considered a useful non-transgenic model for studying aspects of progressive cognitive decline and Alzheimer's disease (AD). Using SAMR1 mice as controls, here we explored the effects of 6 months of voluntary wheel running in 10-month-old female SAMP8 mice. Exercise in SAMP8 mice improved phenotypic features associated with premature aging (i.e., skin color and body tremor) and enhanced vascularization and BDNF gene expression in the hippocampus
more » ... with controls. With the aim of identifying genes involved in brain aging responsive to long-term exercise, we performed whole genome microarray studies in hippocampus from sedentary SAMP8 (P8sed), SAMR1 (R1sed), and exercised SAMP8 (P8run) mice. The genes differentially expressed in P8sed versus R1sed were considered as putative aging markers (i) and those differentially expressed in P8run versus P8sed were considered as genes modulated by exercise (ii). Genes differentially expressed in both comparisons (i and ii) were considered as putative aging genes responsive to physical exercise. We identified 34 genes which met both criteria. Gene ontology analysis revealed that they are mainly involved in functions related to extracellular matrix maintenance. Selected genes were validated by real-time quantitative PCR assays, i.e., collagen type 1 alpha 1 (col1a1), collagen type 1 alpha 2 (col1a2), fibromodulin (fmod), prostaglandin D(2) synthase (ptgds), and aldehyde dehydrogenase (Aldh1a2). As a whole, our study suggests that exercise training during adulthood may prevent or delay gene expression alterations and processes associated with hippocampal aging in at-risk subjects. Organization has stressed the relevance of an active lifestyle to prevent chronic diseases and promote healthy aging [1]. The regular practice of moderate physical exercise enhances cardiovascular health [2] [3] [4] [5] , decreases the impact of risk factors affecting metabolic and brain health [6] [7] [8] [9] [10] [11] , and protects cognitive and psychological functions [12] [13] [14] [15] . There is increasing evidence of the beneficial effects of aerobic physical exercise on synapse and general brain
doi:10.3233/jad-121264 pmid:23168450 fatcat:l2n56ubxd5gljnqpxkybjvdw6m