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A Significant Fraction of Functional SecA Is Permanently Embedded in the Membrane
Journal of Biological Chemistry
SecA has been suggested to cycle on and off the cytoplasmic membrane of Escherichia coli during protein translocation. We have reconstituted 35 S-SecA onto SecA-depleted membrane vesicles and followed the fate of the membrane-associated 35 S-SecA during protein translocation. Some 35 S-SecA was released from the membranes in a translocation-independent manner. However, a significant fraction of 35 S-SecA remained on the membranes even after incubation with excess SecA. This fraction of 35doi:10.1074/jbc.271.47.29698 pmid:8939903 fatcat:smbu5axj2bezfoqgqgumrlxrx4