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The effect of substrate on mycelium growth, the optimal composition of the culture medium, and the optimal cultivation conditions for Streptomyces ornatus S 1220 have been investigated in the present work. The specific activity of keratinase has been monitored during cultivation and activity variation caused by addition of various salts to the cultivation medium has been analyzed. The results of the optimization study are reported and successful use of the culture studied in the present work indoi:10.12737/2051 fatcat:m7252qjxvjcyxotyvbhttivd5a