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Olive is the most diffused fruit crop in the Mediterranean region, and the improvement of its productivity by conventional and biotechnological approaches is currently required. In vitro cultures have been proven to be very useful for this purpose. In this work, callus cultures of olive (Olea europaea L., cv. Raja) were established from basal tissues of nodal explants and subcultured on OM medium in a growth chamber at 25 °C with a 16h-light photoperiod. Cell clusters with differentdoi:10.5296/jab.v3i1.6064 fatcat:k26mv5ltpnbdlpv53jr72r2ajm