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Chitosan(CS) is the unique alkaline polysaccharide in nature, because of its perfect biocompatibility and degradability, it is widely used in medicine, soft release and control release. The betulinic acid is the derivative of betulin, it has many pharmacological activities, such as anti-inflammatory, antitumor, anti-malaria and anti-HIV. In this paper, the Span-80 was used as emulsifiers, the glutaraldehyde was used as crosslinker. With the method of orthogonal experiment, the preparationdoi:10.1051/matecconf/20166303008 fatcat:vkywxneqqja63ojaujjazyokqa