Method of Lumphocytotoxic Crossmatch Test for Feline Renal Transplantation

Yasuhito Kuwahara, Rie Kobayashi, Junko Iwata, Katsuya Kitoh, Hitoshi Kitagawa, Yoshihide Sasaki
1999 Journal of Veterinary Medical Science  
The optimal condition for methods of lymphocytotoxic crossmatch test for feline renal transplantation was investigated. On separation of viable lymphocytes from whole blood, the best results were obtained when Ficoll-diatrizoate with 1.078 of a specific gravity at 20°C was centrifuged with 800 × g for 30 min at 4°C. A nylon wool column was used to separate T and B cells from lymphocyte fraction. The ratio of T cells in nylon wool effluent cells was 95%, while the ratio of B cells in adherent
more » ... ells in adherent cells was 41%. Lymphocytotoxic crossmatch tests were performed by using the effluent cells as T cells and the adherent cells as B cells, at 37°C (warm) and 4°C (cold). The ratio of B cells in adherent cells was low, however, the result was utilized as a matching test before transplantation by combining with the T cell result. The trypan blue stain method made it easier than the eosin stain method to distinguish living and dead cells. The lymphocytotoxic crossmatch tests were performed on 15 pairs of healthy cats, and only one pair showed doubtful positive against anti-B cell cold antibodies. During acute rejection after renal transplantation in two pairs which were negative on any anti-lymphocyte antibodies before the transplantation, the anti-T cell warm antibodies became positive in both pairs, and the anti-T cell cold antibodies became positive on one of the two pairs.-KEY WORDS: anti-lymphocyte antibody, feline, lymphocytotoxic crossmatch test, renal transplantation, trypan blue stain.
doi:10.1292/jvms.61.481 pmid:10379938 fatcat:a5eefuhx5vacdekra734cbls2i