Episomal detection of Banana streak OL virus in single and mixed infection with Cucumber mosaic virus in banana 'Nanicão Jangada'

Patrícia R. Carnelossi, Taise Bijora, Cassiele U. Facco, Jaqueline M. Silva, Marcelo H. S. Picoli, Eliezer R. Souto, Fernando T. de Oliveira
2014 Tropical Plant Pathology  
Banana streak virus (BSV) and Cucumber mosaic virus (CMV) are commonly found in all banana growing-areas of the world. These viruses cause diseases that lead to yield reduction and constrain plant breeding and distribution of Musa germplasm. Most of the diagnostic methods targeting BSV can generate dubious results because of the considerable genetic and serological diversity among BSV isolates and the presence of integrated BSV sequences in the banana plant. Both viruses are usually detected in
more » ... usually detected in single and mixed infections in banana plantations in the north region of Paraná state using DAS-ELISA and PCR. A rolling-circle amplification protocol tested in this study allowed specific detection and identification of an episomal BSV isolate infecting Nanicão Jangada cultivar, thus confirming the occurrence of Banana streak OL virus in Brazil.
doi:10.1590/s1982-56762014000400010 fatcat:piaco626jrhpdl7sut5zoibzyq