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Our study deals with a possibility of determining true protein and casein in cow's, ewe's and goat's milk and in ewe's colostrums by FT NIR spectroscopy. Samples of milk were analysed by FT NIR in the reflectance mode with the transflectance cuvette. The values of correlation coefficients of calibration were as follows: cow's protein 0.943; cow's casein 0.964; ewe's protein 0.997; ewe's casein 0.977; goat's protein 0.989; goat's casein 0.890; ewe's colostrum protein 0.983. Calibration wasdoi:10.17221/2264-cjas fatcat:d6ihkkyqznhkphijzh4s7lomfu