Simultaneous Determination of Eight Ginsenosides in Rat Plasma by Liquid Chromatography–Electrospray Ionization Tandem Mass Spectrometry: Application to Their Pharmacokinetics

Li-Yuan Ma, You-Bo Zhang, Qi-Le Zhou, Yan-Fang Yang, Xiu-Wei Yang
2015 Molecules  
A high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was successfully developed and validated for the identification and determination of eight ginsenosides: ginsenoside Rg 1 (1); 20(S)-ginsenoside Rh 1 (2); 20(S)-ginsenoside Rg 2 (3); 20(R)-ginsenoside Rh 1 (4); 20(R)-ginsenoside Rg 2 (5); ginsenoside Rd (6); 20(S)-ginsenoside Rg 3 (7); and 20(R)-ginsenoside Rg 3 (8) in rat plasma. The established rapid method had high linearity,
more » ... gh linearity, selectivity, sensitivity, accuracy, and precision. The method has been used successfully to study the pharmacokinetics of abovementioned eight ginsenosides for the first time. After an oral administration of total saponins in the stems-leaves of Panax ginseng C. A. Meyer (GTSSL) at a dose of 400 mg/kg, the ginsenosides 6, 7, and 8, belonging to protopanaxadiol-type saponins, exhibited relatively long t max values, suggesting that they were slowly absorbed, while the ginsenosides 1-5, belonging to protopanaxatriol-type saponins, had different t max values, which should be due to their differences in the substituted groups. Compounds 2 and 4, 3 and 5, 7 and 8 were three pairs of R/S epimerics at C-20, which was interesting that the t 1/2 of 20(S)-epimers were always longer than those of 20(R)-epimers. This pharmacokinetic identification of multiple ginsenosides of GTSSL in rat plasma provides a significant basis for better understanding the clinical application of GTSSL.
doi:10.3390/molecules201219790 pmid:26633350 fatcat:flyxlgslwjftnl4qf222akh5uu