Response Gene to Complement-32 Promotes the Imbalance of Treg/Th17 in Patients with Dilated Cardiomyopathy

Bailing Li, Wei Zhou, Xiaojun Tang, Wei Wang, Jiajun Pan, Mengwei Tan
2017 Cellular Physiology and Biochemistry  
Background/Aims: The imbalance of Treg/Th17 cells plays important role in the pathogenesis of dilated cardiomyopathy (DCM). Response gene to complement (RGC)-32 is a cell cycle regulator that plays an important role in cell proliferation. We evaluated whether the upregulation of RGC-32 was implicated in the homeostasis of Treg/Th17 cells in DCM. Methods: The levels of plasma RGC-32, IL-17 and TGF-β1, and the frequencies of circulating CD4 + RGC-32 + T cells, Th17 and Treg cells in patients with
more » ... ls in patients with DCM were determined by Cytokinespecific sandwich ELISA and the flow cytometer (FCM), respectively. Results: A significant elevation of plasma RGC-32 in patients with DCM compared with healthy control (HC) subjects was observed. This upregulation was associated with an increase in frequency of Th17 and a decrease in frequency of Treg cells. To further assessed the role of RGC-32, we investigated the effects of RGC-32 up-or down-regulation on frequencies of Th17 and Treg cells in peripheral blood mononuclear cells (PBMCs) from subjects. Importantly, overexpression of RGC-32 was accompanied by an augmentation of Th17 and a reduction of Treg expression. Conclusion: In summary, our study demonstrated the up-regulation of RGC-32 contributed to the imbalance of Treg/Th17 cells in patients with DCM. Fig. 4. Alterations of RGC-32 expression levels in PBMCs affect the frequencies of Th17 and Treg cells. PBMCs from patients with DCM were transfected with RGC-32-Plasmid or RGC-32-siRNA as described in the Materials and methods section. (A) Western blot analysis of RGC-32 expression in transfected cells. Representative blots of three independent experiments are shown. β-actin was used as a loading control. o/e: overexpression. At 48 h after transfection, and then were challenged with 4μL/mL PMA/ Ionomycin mixture for additional 6 h. Subsequently, Cells were determined (A) expression of Th17 and (B) Treg cells by FCM. Cytokine-specific sandwich ELISA of the culture supernatants was performed to measure (C) the levels of secretory IL-17and (D) TGF-β1. Data are the means of triplicate independent experiments; **p<0.01. Fig.4
doi:10.1159/000481975 pmid:29035886 fatcat:b3ptpk7aofgbph6x7uswrv56g4