Improvement of Technology of Cholera Toxin B-Subunit Production

A. V. Komissarov, S. A. Eremin, O. A. Volokh, O. V. Gromova, Yu. A. Aleshina, E. M. Kuznetsova, N. G. Avdeeva, L. F. Livanova, A. K. Nikiforov
2015 Problems of Particularly Dangerous Infections  
Consideration is given to implementation of state-of-the-art filtration technologies for up-scaled manufacturing of cholera toxin B-subunit, produced by recombinant Vibrio cholerae non O1 KM93 strain. Selected are micro-and ultra-filtration membranes to be incorporated into manufacturing method. Investigated are the properties of cholera toxin B-subunit, obtained applying the pilot technology. The engineered method for up-scaled manufacturing of cholera toxin B-subunit makes the procedure
more » ... -to-maintain due to tangential micro-and ultra-filtration, performed at the stage of purification and concentration. It excludes labor-consuming chromatographic purification, while retaining B-subunit properties. The studies undertaken make it possible to manufacture cholera toxin B-subunit with the same characteristics as in the case of the pilot technology, but under production conditions, and use it as a component for chemical cholera vaccine.
doi:10.21055/0370-1069-2015-4-100-102 fatcat:vtm4apefxrd7rlv3itxlubi4cy