Protective Role of Alginic Acid and Fucoidan from Nitric Oxide-Induced Oxidative Stress

Mi Jung So
2014 Agricultural Science  
The protective activities of alginic acid and fucoidan from oxidative stress (OS) were investigated under cellular system using LLC-PK1 renal epithelial cells that are widely used for study cellular OS. The cellular OS in LLC-PK1 cells was induced by the free radical generators. The treatment of pyrogallol, sodium nitroprusside or 3-morpholinosydnonimine led to the production of superoxide anion (O 2 -), nitric oxide (NO) and peroxynitrite (ONOO -), respectively. The free radical generators
more » ... ical generators decreased cell viability and elevated lipid peroxidation compared OSwith non-treated cells. However, the treatment of alginic acid and fucoidan attenuated OS significantly induced by NO, O 2 and ONOO -, resulting in the significant increase of cell viability and inhibition of lipid peroxidation in concentration-dependent manner. In particular, fucoidan showed the stronger protective effect from oxidative stress than alginic acid. These results indicated that alginic acid and fucoidan played the protective role from NO-induced OS. The present study suggests the promising antioxidative agents of alginc acid and fucoidan with protective activity from free radical-induced OS.
doi:10.12735/as.v2i2p12 fatcat:pf2nrkn6rbfhbpns6xv3eelnia