Expression Profile of Aging and Oxidative Stress Response inDrosophila melanogaster

Sige Zou, Sarah Meadows, Linda Sharp, Lily Y. Jan, Yuh Nung Jan
2001 The Scientific World Journal  
INTRODUCTION. The free radical hypothesis of aging suggests that reactive oxygen species accumulated with age cause oxidative damages to molecules, such as nucleic acids, proteins and lipids, and are causally linked to aging and death(1). The regulation of gene expression in aging and its correlation with that in response to oxidative stress, however, is poorly understood. We have used the microarray technique to study the genome-wide expression patterns of aging and oxidative stress response
more » ... e stress response in Drosophila melanogaster. METHOD. Poly(A) isolated from fly thorax and abdomen was utilized to synthesize fluorescently labeled cDNA. For hybridization to microarrays (2), cDNA from 3-day old flies labeled with Cy3 was co-hybridized with those from 3, 10, 15, 25, 40, or 50 days old flies labeled with Cy5; cDNA from sucrose-fed flies was labeled with Cy3 and co-hybridized with cDNA from 3-hr, 12-hr, 25-hr or 34-hr paraquat-fed flies labeled with Cy5. The microarray data were submitted and stored in the AMAD database designed by J. DeRisi. The expression patterns of all the ESTs were analyzed by the Cluster and Treeview programs (3).
doi:10.1100/tsw.2001.242 pmid:30147616 fatcat:4wcpac5zwvf25bf6gvmlillsou