Dynamics of total proteins during stress alleviation in tomato infected with cucumber mosaic virus
Phytoalexins are the secondary metabolites produced in crop plants after the attack of necrotizing pathogens. They are aimed to support homeostasis in stressed plants. Proteins are also part of this regime that is manipulated according to the type of infection or environmental stress. Chemical elicitors substitute the traditional fungicides and safe to use. They also modify protein levels due to their direct or indirect influence on the defense pathways. Methodology: An in vitro study was
... ted on tomato seedlings inoculated with cucumber mosaic virus (CMV). Plants were treated with salicylic acid and BTH at different intervals to find out the alterations in the total soluble proteins. Purpose of study was to investigate the variation in total proteins during the viral attack and role of elicitors in this case. Results: Results indicated that protein level was initially reduced (133.3 mg) in the inoculated plants prior to the application of elicitors. However, BTH alone or combined with SA significantly enhanced total soluble proteins (200.3 mg) in tomato leaves when applied on weekly intervals. Single application of Salicylic acid gave similar response as in case of non-inoculated plants, however weekly applied SA reduced the total proteins to much lower extent (83.6 mg) under the CMV stress. Discussion: Modification of total proteins in a single tomato cultivar along with homogenous environmental factors indicates the active role of chemical elicitors in the defense pathways. BTH is a structural homologue to SA but expressed a remarkable functional contrast under viral attack particularly in solanaceous crops. This novelty in plant science is worthy of discussion.