A Brief Tip on Decolorizing DAB Staining for Immunohistochemistry

Mike King
2001 Microscopy Today  
section and Epon in between the slides and cure. Our procedure for doing this is: 1) Acid clean and rinse the slides before use. 2) Dry in a drying oven overnight (the type used for drying cleaned laboratory glassware), then let cool to room temperature. 3) Dip slides in the LRA. 4) Stand slides upright and allow to dry in a dust-free area. 5) Tissue was sectioned at 50 to 100 urn in a Vibratome, then processed into 100% epoxy. After the final change, rather than place in molds, place the
more » ... ns on a coated slide, add some Epon, and then a second slide. The section is held flat by putting whatever weight is needed (5 g to 10 g typically; clips may also be used). 6) Place in the curing oven for the recommended time for the epoxy formulation used.
doi:10.1017/s1551929500057011 fatcat:wjzgh47bifempkdimdsxs3czam