UEG Week 2020 Oral Presentations

2020 United European Gastroenterology journal  
In recent years gastrointestinal dysbiosis has been suggested as an important factor in the pathogenesis of inflammatory bowel diseases (IBD). The decrease in bacterial richness as well as changes in the abundance of several bacterial species have been described in patients with IBD, however, the functional and clinical implications of these alterations are still not clear. The study of fecal metabolites is essential for understanding the relations between host and gut microbes, and can
more » ... lly lead to the discovery of new diagnostics and microbiome directed therapies. Aims & Methods: In this study we characterized alterations in the gut microbial metabolism of patients with IBD. We aimed to identify potential new biomarkers for the disease as well as to improve our knowledge on the host-microbiota interaction, considering factors like life-style and clinical history. In short, we have explored the relation between host genetics, gut microbiota and fecal metabolites composition of 500 patients with IBD and 255 population controls. We used Metagenomic shotgun sequencing to profile the microbiota composition, and untargeted metabolomics platform (Metabolon INC., USA) was used to identify the levels of 1684 fecal metabolites including eight short chain fatty acids. Whole exome sequencing and GSA Illumina chip were used to characterize host's genetics. Associations between multiple data layers were performed using multivariate linear regression correcting for age, sex and technical covariates such as sample storage time and day of the experiment. Significant associations were considered after Bonferroni multiple testing adjustment. Results: We found that the levels of 597 fecal metabolites were altered in patients with Crohn's disease (CD) and 209 in patients with ulcerative colitis (UC) as compared to the population controls (adjusted p-value< 0.05). The level of metabolites related to the sphingolipid synthesis was increased in both CD and UC samples, while fatty acid metabolites were
doi:10.1177/2050640620927344 pmid:33043828 pmcid:PMC8939486 fatcat:m5vmaqy3cfflplneadhkhihjla