Plasmodium vivax: Chloroquine Drug Resistance in Strains Isolated from Navi Mumbai, Maharashtra, India
MGM Journal of Medical Sciences
Aim: Malaria imposes a significant public health burden worldwide. Chloroquine (CQ) resistance has been shown to be associated with point mutations in Plasmodium vivax chloroquine resistance transporter (Pvcrt) and Plasmodium vivax multidrug resistance transporter (Pvmdr1). The present study was carried out to study the association of Pvcrt-o K10 (lysine) insertion and Pvmdr1 Y976 mutations with CQ resistance in Northeast Indian Plasmodium vivax isolates. Materials and methods: The study was
... s: The study was conducted in the Parasitology Laboratory at the Department of Microbiology, Mahatma Gandhi Mission Medical College and Hospital, Kamothe, Navi Mumbai, Maharashtra. A total of 22 Plasmodium vivax isolates were subjected to the in vitro CQ-sensitivity test and the polymerase chain reaction (PCR) test for the Pvmdr1 Y976 and Pvcrt-o K10 (lysine) insertion mutations. Result: Five isolates of Plasmodium vivax were found to be resistant to CQ by the in vitro antimalarial drug-sensitivity test, while 17 were found to be CQ sensitive. All the CQ-resistant isolates showed the presence of Pvmdr1 and Pvcrt mutations. CQ-sensitive isolates were negative for these mutations. Strong linkage disequilibrium was observed between the alleles at these two loci [Pvmdr1 Y976 and Pvcrt-o K10 (lysine) insertion]. Conclusion: Our study supports the use of molecular methods for the detection of Pvmdr1 Y976 and Pvcrt-o K10 (lysine) insertion mutations to identify CQ drug resistance in Plasmodium vivax and to provide early and proper treatment to patients suffering from vivax malaria.