Genetic Association of Interferon Gamma Induced Protein-10 (IP-10), CXCL-10 Gene Polymorphisms with TB Pleurisy Susceptibility in South Indian Population

Ghousunnissa Sheikh, Venkata Sanjeev Kumar Neela, Satya Sudheer Pydi, Naveen Chandra Suryadevara, Ramulu Gaddam, Suman Latha Gaddam, Sai Kumar Auzumeedi, Vijaya Lakshmi Valluri
2015 Open Journal of Immunology  
CXCL-10 known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine 10 is a 8.7 kDa protein, which is secreted in response to IFN-γ by monocytes, endothelial cells and fibroblasts. It has chemo-attraction for monocytes/macrophages, T cells, NK cells and dendritic cells in promotion of T cell adhesion to endothelial cells. In the present study, we investigated whether polymorphisms in CXCL-10 gene have any role in the manifestation of Tuberculous (TB) pleurisy. Two SNPs in
more » ... risy. Two SNPs in CXCL-10 promoter region (−1447A > G and −135G > A) were genotyped in patients with TB Pleurisy (n = 186), Pulmonary TB patients (n = 159) and healthy controls (n = 205) by PCR-RFLP. Disease associations were statistically analyzed by Fisher exact test. At the −135G > A position, the frequencies of genotype GA and allele G were significantly high in TB pleurisy patients compared to healthy controls. While the frequencies of genotype AA and allele A were significantly low in TB pleurisy patients compared to healthy controls. The frequency of haplotype A-G with the combination of 1447A > G and −135G > A was significantly high in TB pleurisy. Our results reveal that genotype GA and allele G at −135G > A position were strongly associated with susceptibility to tuberculous pleurisy. The GA genotype may be a useful genetic marker for early detection of the disease in high risk individuals.
doi:10.4236/oji.2015.52008 fatcat:36xe7anwinbpfj5dhqmtwvfeza