EFFECT OF UNDER CONVENTIONAL SOLVENT EXTRACTION ON POLYPHENOL CONCENTRATIONS AND ANTIOXIDANT ACTIVITIES IN Thespesia lampas

Sunayana Nath, Bhawana Pathak.
2017 International Journal of Advanced Research  
Objectives: This study aimed to evaluate the polyphenol content and antioxidant activity in dried leaf, fruit, root and flower of Thespesia lampas Dalz. and Gibs (Cav.) extracted in acetone, isopropanol, acetonitrile and dicholoromethane (under conventional solvents). Material and methods: The antioxidant profile was evaluated using total antioxidant capacity (TAC), ABTS and DPPH assay, Fe 2+ chelating ability, iron reducing power (RP) in vitro assays. Results: Highest total phenolic content
more » ... phenolic content (TPC) and flavonoid were noted in acetone extracted flower (Fac) and dichloromethane extracted root (Rdcm) respectively. Fac showed maximal activity for TAC (212.16 µM TE/ mg DW), ABTS (IC 50 = 1.12 ± 0.00 mgmL -1 ), DPPH (IC 50 = 0.87 ± 0.01 mgmL -1 ) and RP (IC 50 = 67.48 ± 0.54 mgmL -1 ) while Rdcm showed highest Fe 2+ chelating ability (IC 50 = 1.70 ± 0.05 mgmL -1 ). Acetone emerged as the most efficient solvent in extraction of antioxidants among the solvents exercised. A positive correlation was observed between TPC and ABTS (R 2 = 0.952), TPC and TAC (R 2 = 0.881), ABTS and TAC (R 2 = 0.892); and DPPH and RP (R 2 = 0.688) assays.
doi:10.21474/ijar01/5698 fatcat:npag7an5jfewronczgogbbvuvu