Blood, sweat and tears: non-invasive vs. non-disruptive DNA sampling for experimental biology [post]

Marie-Caroline Lefort, Stephane Boyer, Arijana Barun, Arsalan Emami Khoyi, Johnathon Ridden, Victoria R Smith, Rowan Sprague, Benjamin R Waterhouse, Robert H. Cruickshank
2015 unpublished
DNA data are becoming increasingly important in experimental biology. For example, it may be necessary to obtain DNA from an organism before using it in a bioassay or an experiment, to identify and distinguish between cryptic species, or when comparing different morphocryptic genotypes. Another example could be the assessment of relatedness between organisms prior to a behavioural study. In such cases, DNA must be obtained without affecting the fitness or behaviour of the subject being tested,
more » ... ject being tested, as this could bias the results of the experiment. This points out the existence of a gap in the current molecular and experimental biology terminology, for which we propose the use of the term non-disruptive DNA sampling, specifically addressing behaviour and/or fitness, rather than simply physical integrity (invasiveness). We refer to these methods as "non-disruptive", and discuss when they are appropriate to use.
doi:10.7287/peerj.preprints.655v3 fatcat:3jjifryo75bc5dn4kkvkzjjoqu