Antiangiogenic and Apoptotic Effects of Oleuropein on Breast Cancer Cells

Recep Liman, Funda Çoban, Ibrahim Ciğerci, İbrahim Bulduk, Selahattin Bozkurt
2017 British Journal of Pharmaceutical Research  
Oleuropein is a fundamental polyphenol contained in olive fruit, oil, and leaf, with a bitter taste. A decrease in its level is observed as the fruit ripens. Leaves of oil tree were used in this study as a possible origin of oleuropein. In this study, cytotoxic, genotoxic, apoptotic and antiangiogenic impacts of isolated oleuropein (0.5xIC 50 , IC 50 and 2xIC 50 ) on MCF-7 human breast adenocarcinoma cells were examined by means of 3-[4,5-Dimethylthiazol-2-yl] 1-2,5diphenyltetrazolium bromide
more » ... TT) kit, Comet assay, Poly (ADP-Ribose) Polymerase (PARP) cleavage ELISA Kit and Vascular Endothelial Growth Factor (VEGF) Human ELISA Kit for 24, 48 and 72 h, respectively. The IC 50 value of oleuropein on MCF-7 cells was found to be 11.02±0.52, 9.43±0.41 and 6.81±0.18 µg/mL for 24, 48 and 72 h, respectively. The time and concentrationrelated cytotoxic impacts of oleuropein were determined to be significant. Cleaved PARP level was Original Research Article significantly increased especially with an increase in the concentration of oleuropein. However, a significant decrease in VEGF level was observed (p<0.05). Furthermore, oleuropein increased damage on DNA for 48 and 72 h. Findings showed oleuropein as a potential antiangiogenic, apoptotic and genotoxic agent for the breast cancer cells.
doi:10.9734/bjpr/2017/33403 fatcat:wyajub4fyva4djcx62ns3elmri