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Zinc finger protein X-linked (Zfx) was regarded to be a sex determination factor, and plays a critical role in spermatogenesis. RNAi is an effective method of silencing Zfx/Zfy mRNA expression. However, there has been little research on the use of RNAi technology to control the sex of the offspring of Cervus elaphus. The objective of this study was first to explore an efficient method to alter the Cervus elaphus offspring sex-ratio by silencing the genes Zfx during spermatogenesis. Results:doi:10.21203/rs.2.19002/v1 fatcat:zhnvydmmonh2fatsv4os6ewlxa